Induction of IL-6 and IL-1β in response to demand is impaired in Eif2b5^R132H/R132H mice brain.

<p>Intraperitoneal injections of 5 mg/kg LPS were administered to 4–6 four-weeks-old Eif2b5^R132H/R132H (Mut) and wild-type (WT) mice. At 6 h post-injection, mice were sacrificed and brains were removed. (A) Representative immunoblot analysis using anti-TLR4 and anti-β-actin antibodies. Data represent the average of TLR4/β-actin level of 3 mice per group ± SEM. (B) Total RNA was extracted and subjected to qRT-PCR analysis of IL-6 and IL-1β mRNA levels. Bars represent the average of 5 mice per group, normalised to untreated WT±SEM. No significant (NS) differences between Mut and WT were observed. (C) Representative immunoblot analyses using anti-IL-6, anti-IL-1β and anti-β-actin. Data represent the average of IL-6/β-actin and IL-1β/β-actin of 3-6 mice per group ±SEM relative to WT.