p300 facilitates parental trans-generational transmision of remembered states of potentiated transcriptional function.

<p>(A) Schematic of the experimental design. Jurkat cells were treated with PMA and Ionomycin (P/I) for 1 h. Cells were then washed and allowed to rest for 40 h. Western blots showing protein level of phospho-(Thr202/Tyr204) ERK and phospho- (Ser133) CREB at the respective time point. Shown is 1 of 2 independent biological replicate. (B) qRT-PCR profile showing expression of <i>FOS</i> expression from Jurkat cells pre-treated with P/I (mitogen pulsed) for 1 h, washed, allowed to rest for 40 h and restimulated with P/I (1 h) and TSA (2 h) (left). The amount of fold induction present upon TSA stimulation is presented as relative level compared to the amount present at the unstimulated population (right). (C) Flow cytometric (FACS) analysis of CFSE with Jurkat cells comparing control and P/I washout cells to indicate cell proliferation or cell division. (D) Position dependent profile at the <i>FOS</i> locus for Jurkat cells at indicated position (relative to TSS) for indicated antibodies as determined by quantitative ChIP analysis. Error bars represent standard error of mean from 2 biological replicates each determined in duplicate. (E) Position dependent profile at the <i>FOS</i> locus for the p300 WT and p300 KO in HCT 116 cells were used. Error bars represent standard error of mean from 2 biological replicates each determined in duplicate.