NFAT mRNA and protein expression in 1HAE₀ cells after estradiol stimulation for 24 h.

<p>A) Baseline mRNA expression of NFAT (c1–c4) in 1HAE₀ cells quantified by real-time PCR expressed as absolute change in C_t value normalized to HPRT1. B) NFATc1–c4 mRNA expression normalized to HPRT1 in cells treated with 10⁻⁷ M estradiol for 24 h. Data are expressed as fold increase over vehicle controls. Values shown are mean ± SEM of experiments performed in 3 independent experiments. ****P<0.0001 compared against vehicle control in each gene using non-parametric t-test in B-C.