Effect of low dose IGF1 on <i>A. stephensi</i> survivorship was ablated by enhanced oxidative stress.

<p>Mosquitoes were fed saline/ATP supplemented with: (1) low (0.13 µM) IGF1 or high (0.13 µM) IGF1 and 1 mM paraquat (P), (2) an equivalent volume of PBS and 1 mM paraquat (PBS+P), or (3) an equivalent volume of PBS without paraquat (PBS−P) as an untreated control. Dead mosquitoes were removed and counted at intervals between 8 h and 72 h. The experiment was replicated twice with separate cohorts of mosquitoes. Survival analyses were performed using the Kaplan Meier method and differences between survival curves calculated using the Wilcoxon test. Survival curves for 0.13 µM IGF1+P and 0.13 µM IGF1+P were not significantly different from each other and neither was significantly different from PBS+P (p>0.05). Survival curves for low IGF1+P, high IGF1+P, and PBS+P, however, were significantly different from the untreated control PBS−P (p<0.05).