TLR8 stimulation mimics MAIT cell activation upon bacterial infection.

<p>(<i>Upper panels</i>) Representative contour plots illustrate the production of IFN-γ and TNF-α by MAIT cells upon overnight stimulation of liver-derived cells with ssRNA40 (5 µg/mL), riboflavin-synthesizing bacteria (<i>Pseudomonas aeruginosa</i>), anti-CD3/CD28-coupled beads or PMA/Ionomycin. (<i>Lower panels</i>) Pie charts depicting the proportion of IFN-γ or TNF-α monoproducers and IFN-γ/TNF-α double producers among activated MAIT cells are shown. Data expressed as means obtained from two healthy donors.