MAPK inhibitors, Vit. C, and PDTC inhibited the LPS induced degradation of IκB-α.

<p>Cultured cells were treated for 60(control), LPS alone (100 ng/ml), or simultaneous LPS with SB203580 (20 µM), PD98059 (20 µM), Vit. C (10 mM), or the NF-κB inhibitor PDTC (50 µM) and then harvested to conduct western blot analyses of the IκB-α degradation. The LPS-induced suppression of IκB-α degradation was significantly inhibited by cotreating all inhibitors and Vit. C, which exerted the least amount inhibition. The data are expressed as the mean ± SEM based on 4–5 experiments. ** <i>p</i><.01 and *** <i>p</i><.001 versus Cont.; ⁺<i>p</i><.05, ⁺⁺⁺<i>p</i><.001 versus LPS alone.