The effects of inhibitors of the downstream targets of FGF and VEGF signaling pathways on compound 10-induced angiogenesis in VRI-treated zebrafish.

<p>(I) 24 hpf zebrafish embryos were treated with 0.1% DMSO (v/v)(A),10 µM PP2 (D), 10 µM LY294002 (F),5 µM P38i (H), 50 µM Rafi (J),20 µM MEK1/2 (L),25 µM ERK1/2i (N) for 24 h. 21 hpf embryos were treated with VRI (500 ng/ml) for 3 h. After that, the VRI was washed out and replaced with 0.1% DMSO (v/v) embryo medium (B) or 0.3 µM <b>10</b> (C), 0.3 µM <b>10</b> and 10 µM PP2 (E), 0.3 µM <b>10</b> and 10 µM LY294002 (G), 0.3 µM <b>10</b> and 5 µM P38i (I), 0.3 µM <b>10</b> and 50 µM Rafi (K), 0.3 µM <b>10</b> and 20 µM MEK1/2i (M), 0.3 µM <b>10</b> and 25 µM ERK1/2i (O) for another 24 h. Yellow arrows indicated that the ISVs (intact and defective), SIVs, DLAVs, and DA. (II) Quantitative analysis of inhibitory effect of inhibitors of the downstream targets of FGF and VEGF signaling pathways. Data are expressed as mean±SEM, (n≧3). *p<0.05, **p<0.01 and ***p<0.001 versus the 10 treatment group.