Dose-dependent effect of cytokines on ROS generation in HBMvECs.

<p>Confluent cells were treated with TNF-α (<b>A</b>) or IL-6 (<b>B</b>) (0–100 ng/ml, 6 or 18 hrs) and ROS generation monitored by flow cytometry using fluorescent ROS-detecting compounds, DHE (PE Texas Red detector) or CFDA (FITC detector). Histograms (LHS) represent the fold change in fluorescent signal normalized to untreated DHE control at 6 or 18 hrs. Representative FACS scans (RHS) are shown for both 6 and 18 hr treatments with increasing cytokine concentration evident from left to right. Grey shaded scan indicates untreated control (full key beneath scans). *<i>P</i>≤0.05 versus untreated DHE 6 or 18 hr controls.