Effect of NADPH oxidase blockade on cytokine-induced ROS generation in HBMvECs.

<p>Confluent cells were either transfected with siRNA targeting gp91 or p47, or were pre-treated with NSC23776 (50 µM) for 1 hr prior to stimulation with TNF-α (<b>A</b>) or IL-6 (<b>B</b>) (100 ng/ml, 6 or 18 hrs). ROS generation was subsequently monitored by flow cytometry using ROS-detecting CFDA. Histograms (LHS) represent the fold change in fluorescent signal normalised to untreated control at 6 or 18 hrs. Representative FACS scans (RHS) are shown for both 6 and 18 hr treatments. Grey shaded scan indicates untreated control (full key beneath scans). *<i>P</i>≤0.05 versus untreated control at 6 or 18 hrs. ^Ø<i>P</i>≤0.05 versus cytokine without NADPH oxidase blockade.