CMHX008 differentially regulates PPARγ target genes in 3T3-L1 adipocytes.

<p>Post-confluent 3T3-L1 preadipocytes were incubated in differentiation medium for 2 days. The medium was changed to DMEM containing insulin and 10% FBS for another two days and then replaced again with fresh DMEM containing 10% FBS for another 3 days. Rosiglitazone and CMHX008 were administered at the initiation of differentiation and with every medium change. At day 7, the differentiation cells were harvested for total RNA extraction and the mRNA expression of adiponectin (A), Glut4 (B) and aP2 (D) was analyzed by real-time PCR, and secreted adiponectin in the culture medium was measured using adiponectin ELISA kit (B). Data are the means ± S.E.M. from six independent experiments. *: <i>P<</i>0.05; NS: no significance.