Effects of PPARγ antagonists on rosiglitazone-induced adipocyte differentiation.

<p>Primary bone marrow cells were treated with BADGE (20 μM) or GW9662 (1 μM) in the presence of rosiglitazone (10 μM) for 12 days. Osteoblast differentiation was determined by Alizarin red staining (A), and the mRNA levels of PHD1, 2, and 3, and osteogenic marker genes were analyzed by RT-PCR (B). Densitometric analysis was conducted using UN-SCAN-IT gel ver. 5.1 software (Silk Scientific) and is expressed as means ± SEMs. *<i>P</i><0.05 vs control (C), #<i>P</i><0.05 vs differentiated (D), ✝<i>P</i><0.05 vs DR. Experiments were conducted three times and representative results are shown. C, control (no differentiation); D, differentiated in DAG medium; DR, DAG in the presence of rosiglitazone; BD, BADGE; GW, GW9662.