Structures and surface electrostatic characteristics of DUF1471 proteins.

<p><b>A</b>: SrfN (dimer), <b>B</b>: YahO, <b>C</b>: SssB-I, <b>D</b>: SssB-III. In addition to ribbon cartoons colored by secondary structure, NMR structures show 20-member ensembles as Cα traces superimposed over the ordered residues determined by PSVS <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101787#pone.0101787-Bhattacharya1" target="_blank">[51]</a>. Electrostatic surface calculations were calculated with APBS using the PyMol plugin, with default parameters (0.15 M salt, kT/e = −1 to 1, pH 7.0) for the first members of the SrfN, SssB-I, and YahO ensembles and for SssB-III after conversion with PDB2PQR. The van der Waals surfaces are shown and are colored according to the charge (red for negative, blue for positive) on the water-accessible surface. Hydrogen atoms were added to SssB-III. The C-terminal 6x-His tags were removed and replaced with carboxylates for the calculation. N-termini for SrfN and YahO did not have the cleaved signal sequence, the N-terminus of SssB-III began at residue Lys 247.