Analysis of <i>lip5</i> mutants for responses to a virulent <i>Pst</i>DC3000 strain.

<p>(<b>A</b>) Disease symptom development in the <i>lip5</i>, <i>sid2</i> and <i>npr1</i> mutants. Wild type (WT) and mutant plants were infiltrated with a suspension of <i>Pst</i>DC3000 (OD₆₀₀ = 0.0002 in 10 mM MgCl₂). (<b>B</b>) Increased bacterial growth in the <i>lip5</i>, <i>sid2</i> and <i>npr1</i> mutants. Pathogen inoculation of wild-type and mutant plants was performed as in <b>A</b>. Samples were taken at 0 or 4 dpi to determine the bacterial growth. (<b>C</b>) Disease symptom development in complemented <i>lip5</i> mutant plants. WT and <i>lip5-1</i> mutant plants complemented with an empty vector (−) or with various <i>LIP5</i> genes were infiltrated <i>Pst</i>DC3000 as in <b>A</b>. (<b>D</b>) Bacterial growth in complemented <i>lip5</i> mutant plants. Pathogen inoculation and determination of bacterial growth were performed as in <b>B</b>. Pictures of representative inoculated leaves in <b>A</b> and <b>C</b> were taken at 4 dpi. The means and standard errors in <b>B</b> and <b>D</b> were calculated from 10 plants for each mutant. According to Duncan's multiple range test (P = 0.05), means of colony-forming units (cfu) do not differ if they are indicated with the same letter.