<i>aterf-1</i> mutant shows increased susceptibility to bacterial infection.

<p>(<b>A</b>) Macroscopic symptoms of disease caused by inoculation of <i>Pst</i> strain into the leaves of Col-0 and <i>aterf-1</i> mutant. (<b>B</b>) Rossette leaves of 5-week-old plants were inoculated with a <i>Pst</i> suspension in series of 8 plants per genotype. Disease symptoms (3 days post-inoculation) were rated on 75–88 leaves/genotype comparing leaves of similar developmental state. The following disease severity classes were established: I, 0–10% of leaf surface as a chlorotic lesion, II, 10–50% of leaf surface, and III, >50% of leaf surface. The percentage of leaves falling in each category is represented with the number of scored leaves inside the bars (<b>C</b>) Disease Index (DI) was calculated for each genotype according to the formula DI = (0<i>n</i>₁+1<i>n</i>₂+2<i>n</i>₃)/<i>N</i>_t where <i>n</i>₁–<i>n</i>₃ is the number of leaves in the I, II or III classes and <i>N</i>_t is the total number of leaves collected from one plant. Asterisk indicates significant differences respect to the wild-type genotype with a p = 3.15×10⁻⁶ (<i>t</i>-test) (<b>D</b>) Bacterial titre in <i>Pst</i>-inoculated leaves at 3 and 4days post-inoculation (dpi). Bars represent the average cfu/cm² leaf surface and error bars represent SD. Asterisks indicate significant difference between genotypes (p<0.01), which was observed in two independent bioassays.