Tests for neutrality for each gene.

<p><i>Tajima's D, and Fu and Li's D* and F* were calculated using the online Intrapop Neutrality Test calculator (</i><a href="http://wwwabi.snv.jussieu.fr/achaz/neutralitytest.html" target="_blank">http://wwwabi.snv.jussieu.fr/achaz/neutralitytest.html</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101389#pone.0101389-Achaz1" target="_blank">[<i>38</i>]</a><i>). N = # samples; S = # segregating sites, i.e. polymorphic nucleotides; θ_π = #mutations (theta) per site from pi (nucleotide diversity); and θ_S = theta per segregation site. Bold P-values indicate statistical significance based on 100,000 replicates with no recombination, and normalized by their standard deviations. Significant positive D/D*/F* indicates balancing selection or bottleneck; significant negative D/D*/F* indicates directional or expansion</i>.