Purple/white fold-change comparison of mRNA-Seq and quantitative real time PCR (qRT-PCR) expression estimates.

<p>For both methods, chalcone synthase (CHS) had the greatest expression difference between purple- and white-flowered samples among nine genes in the anthocyanin biosynthetic pathway (Savage River = filled triangles; 12 Mile Summit = open circles). The remaining anthocyanin biosynthetic pathway genes have comparable expression between purple- and white-flowered samples and show a significant correlation between mRNA-Seq and qRT-PCR expression estimates (inset).