Enrichment of schizonts from cells synchronised in S-phase or M-phase.

<p>A: Overview of the workflow: TaC12 cells were synchronised in S-phase (thymidine block) or mitosis (nocodazole block). Schizonts were enriched, lysed, and analysed directly by LC MS/MS or after TiO₂ enrichment of phosphopeptides. Raw data were analysed using Progenesis and PEAKS. B: Schizonts were enriched using a Nycodenz step gradient. The sample is shown before (left) and after (right) centrifugation. The fraction containing enriched schizonts is indicated with an arrow. C: Western blot analysis of whole cell lysates (1) compared to purified schizonts (2). Equal amounts of protein from whole TaC12 lysates and enriched schizonts purified from S-phase and mitosis synchronised cells were subjected to Western blotting using an anti-<i>Theileria</i>-HSP70 antiserum to detect the parasite and anti-tubulin to detect host cell tubulin.