Western blotting analysis of TcSMP proteins in the different developmental stages of <i>T</i>. <i>cruzi</i> and in the procyclic form of <i>T</i>. <i>brucei rhodesiense</i>.

<p>A) Samples of <i>T</i>. <i>cruzi</i> (CL strain) (1 x 10⁷ cells/slot) and <i>T</i>. <i>brucei</i> (5 x 10⁶ cells/slot) were lysed in Laemmli sample buffer. Proteins were separated by electrophoresis on SDS- polyacrylamide gel (10%), transferred to a nitrocellulose membrane and reacted against anti-TcSMP polyclonal antibodies. Antibody against a constitutively expressed protein (tubulin) was used as a loading control. <i>T</i>. <i>cruzi</i> developmental forms: epimastigotes (E), metacyclic trypomastigotes (M), extracellular amastigotes (A) and tissue culture trypomastigotes (T). <i>T</i>. <i>brucei rhodesiense</i> procyclic forms. B) Two μg of the recombinant TcSMP-GST purified protein was separated by SDS-PAGE and reacted against anti-TcSMP and anti-GST antibodies. The recombinant protein is coded by a 311-bp fragment of TcSMP in phase with GST (see <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004216#sec005" target="_blank">methods</a> and <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004216#pntd.0004216.s004" target="_blank">S4 Fig</a>). Molecular mass markers in kilodaltons (kDa) are indicated on the left and reactive proteins molecular mass on the right.