LC3-associated phagocytosis (LAP) by Granulocytes.

<p>Non-florescent carboxyl modified beads were injected into the hemocoel. GFP LC3 was used for detection of autophagosome formation in granulocytes at 0–96 h. (A–G) DIC microscope images and fluorescent microscope images (A1–G1) of granulocytes stained with DAPI and GFP-LC3; and flow cytometric analysis (A2–G2). (H) Confocal images of granulocytes stained with DAPI and GFP LC3 at 24 h post injection. (H1) Representative histogram overlay (filled histogram, 0 h post infection, A2 h post infection, A2; green transparent histogram, 24 h post infection, (E2). Based on the green fluorescence intensity, two peaks were identified, LC^low and LC^high. (A2 and E2) 11.40% of granulocytes at 24 h post infection and 1.39 h post infection and 1.39% of granulocytes at 0 h post infection were in the LC h post infection were in the LC^high region, and approximately 10% of GFP-stained hemocytes were increased in the LC^low region at 24 h post infection. (F2 and G2) At 48 and 96 h post infection, LC3 accumulation in granulocytes decreased gradually. h post infection, LC3 accumulation in granulocytes decreased gradually.