Granulocyte mitosis induced by non-florescent carboxyl modified beads injection into the hemocoel.

<p>(A) Analysis by DIC and fluorescent microscopes showed that the granulocytes were mitotically divided at 96 h post injection. (B) Flow cytometric analysis of granulocyte mitosis. Shown are representative histogram overlays: filled histogram (0 h injection h injection) and blue transparent histogram (96 h post injection h post injection). 2.42% of hemocytes in naïve larva were undergoing mitosis, compared to 8.60% of hemocytes in challenged larvae at 96 h post injection. h post injection.