Postnatal decrease in PV+ cortical interneurons in Fgfr1 mutant mice.

<p>PV and GFP immunostaining in 8 week old <i>Fgfr1^f/f;Gad67-GFP+</i> control (<b>A</b>, n = 5) and <i>Fgfr1^f/f;hGFAP-Cre;Gad67-GFP+</i> mutant mice (<b>B</b>, n = 9). Scale bar is 50 µm in <b>A</b>,<b>B</b>. Stereological analysis show significant decreases in in both GFP and PV immunopositive cells in Fgfr1 mutant mice as compared to littermate controls (<b>C</b>). Immunostaining for GFAP, Neun, Ng2, Olig2 and GFP in cortex of adult TgFgfr1-GFP mice (<b>D, E, F</b>). Cortical GFAP+ cells colocalized with strong GFP expression (arrows), while some Neun+ cells (double arrows) also colocalized with weak GFP (<b>D</b>). NG2 positive cells (arrows), and some Olig2 positive cells (double arrows) also colocalized with weak GFP (<b>E</b>). PV immunoreactivty did not colocalize with GFP (<b>F</b>, scale bar is 10 µm and 20 µm in <b>G</b>). In embryonic brain, GFP was highly expressed in the hippocampal Hem and choroid plexus, but was very low in the MGE (<b>G</b>, insert shows coronal section and region imaged). acc = anterior cingulate cortex acc, MGE = medial ganglionic eminence, Hem = hippocampal hem, Ch. Pl = choroid plexus.