Western blot analysis.

<p>Western blot analysis of proteins extracted from mature spores of wild type (PY79, lane 1), <i>ΔcotGΔcotH</i> (AZ603, lane 2), <i>ΔcotGΔcotH amyE::cotGcotH</i> (AZ608, lane 3), <i>ΔcotGΔcotH amyE::cotG_stopcotH</i> (AZ604, lane 4), <i>cotH::spc</i> (ER220, lane 5) and <i>ΔcotGΔcotH amyE::cotG</i> (AZ607, lane 6 of panel B) strains. For CotA and CotB detection (panel A) the proteins have been extracted by SDS treatment while for CotC and CotU detection (panel B) the NaOH treatment has been used. Proteins (25 µg) were reacted with CotA, CotB and CotC specific rabbit antibodies and then with peroxidase-conjugated secondary antibodies and visualized by the Pierce method. The estimated size of CotB, CotC and CotU is indicated.