Western blot analysis using antibodies against 6-Cys A, and HAP2, performed on non-induced (NIC) and induced (IC) <i>B</i>. <i>bovis</i> parasites developed in <i>in vitro</i> cultures.

<p>Monoclonal RAP-1 antibodies were used to detect RAP-1 protein during <i>in vitro</i> cultured <i>B</i>. <i>bovis</i> as a positive control, and pre-immune rabbit serum as a negative control PI rabbit serum. Size markers (M) in kDa are indicated at the left side.