<p>The oligonucleotides chosen for amplification and detection of IS<i>6110</i> and IS<i>1081</i> are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103091#pone-0103091-t002" target="_blank">Table 2</a>. F = dT-FAM, H = tetra hydrofuran and Q = dT-Black Hole Quencher 1.</p
Primers and probes oligonucleotides for real-time-PCR assays used in this study.</p
<p>Sequence of primers used for generation of the MARX_S29 fragment by PCR, quantitative PCR for det...
a<p> = PCR primers (F = forward; R = reverse)</p>b<p> = Taqman primers</p>c<p> = Taqman probe</p>d<p...
<p>Oligonucleotide primers used in this study <a href="http://www.plosone.org/article/info:doi/10.13...
a)<p>F, forward primer; R, reverse primer; P, probe; FAM, 6-carboxyfluorescein; BHQ1, Black Hole Que...
<p>*qHSP-F and qHSP28.6-R were used for the selective amplification of HSP28.6;</p><p>qHSP-F and qHS...
<p>The letters in the first column refer to the primer binding sites indicated in <a href="http://ww...
<p><sup>a</sup> probes have 3′ Iowa Black Quencher and an an internal second quencher ZEN (IDT).</p
<p>The different fluorescent labels on the 5’ end (FAM/HEX/TEXAS RED/CY5) and compatible black hole ...
<p><sup>1</sup>The nucleotide numbering refers to the numbering from the Tohama I genome [<a href="h...
<p><sup>a</sup>[<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003765#pntd....
<p>The nucleotide sequences of the sequencing primers were attached as a binding site to the 5′ end ...
<p>Bold: T7 and UP extension, respectively.</p><p>Abbreviations: FAM, carboxyfluorescein; CY, cyanin...
<p>Anchor and capture probes, and synthetic oligonucleotides target regions used in the MAMEF assay ...
<p><i><sup>a</sup></i>BHQ1, black hole quencher-1; 6FAM, 6-carboxyfluorescein; JOE, 6-carboxy-4',5'-...
Primers and probes oligonucleotides for real-time-PCR assays used in this study.</p
<p>Sequence of primers used for generation of the MARX_S29 fragment by PCR, quantitative PCR for det...
a<p> = PCR primers (F = forward; R = reverse)</p>b<p> = Taqman primers</p>c<p> = Taqman probe</p>d<p...
<p>Oligonucleotide primers used in this study <a href="http://www.plosone.org/article/info:doi/10.13...
a)<p>F, forward primer; R, reverse primer; P, probe; FAM, 6-carboxyfluorescein; BHQ1, Black Hole Que...
<p>*qHSP-F and qHSP28.6-R were used for the selective amplification of HSP28.6;</p><p>qHSP-F and qHS...
<p>The letters in the first column refer to the primer binding sites indicated in <a href="http://ww...
<p><sup>a</sup> probes have 3′ Iowa Black Quencher and an an internal second quencher ZEN (IDT).</p
<p>The different fluorescent labels on the 5’ end (FAM/HEX/TEXAS RED/CY5) and compatible black hole ...
<p><sup>1</sup>The nucleotide numbering refers to the numbering from the Tohama I genome [<a href="h...
<p><sup>a</sup>[<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003765#pntd....
<p>The nucleotide sequences of the sequencing primers were attached as a binding site to the 5′ end ...
<p>Bold: T7 and UP extension, respectively.</p><p>Abbreviations: FAM, carboxyfluorescein; CY, cyanin...
<p>Anchor and capture probes, and synthetic oligonucleotides target regions used in the MAMEF assay ...
<p><i><sup>a</sup></i>BHQ1, black hole quencher-1; 6FAM, 6-carboxyfluorescein; JOE, 6-carboxy-4',5'-...
Primers and probes oligonucleotides for real-time-PCR assays used in this study.</p
<p>Sequence of primers used for generation of the MARX_S29 fragment by PCR, quantitative PCR for det...
a<p> = PCR primers (F = forward; R = reverse)</p>b<p> = Taqman primers</p>c<p> = Taqman probe</p>d<p...