Transcriptional regulation of endogenous target genes by PML/RARA mutants.

<p>(A-B) NIH-3T3 cells stably expressing the indicated PML/RARA mutants were treated or not with 10⁻⁶ M ATRA overnight. <i>RARB</i> and <i>Tg II</i> gene expression were analyzed by quantitative RT-PCR. (C-G) The indicated mutant PML/RARA-transduced primary hematopoietic progenitors were treated with or without 10⁻⁶ M ATRA for 14 hours. <i>RARB</i>, <i>Tg II</i>, <i>HCK</i>, <i>PU.1</i> and <i>ID1</i> gene expression was also analyzed by quantitative real-time PCR. All results from quantitative real-time PCR were normalized to <i>β-actin</i>. Results, obtained from three independent experiments, are given as mean ±standard deviations.