DNA-binding defective PML/RARA mutants disrupt PML nuclear bodies.

<p>NIH-3T3 cells (A and C) or mouse HPCs (B and D) were infected with retroviral constructs expressing wild type or the indicated mutant PML/RARA respectively. Immunofluorescence analysis (A and B) was carried out with a rabbit anti-PML specific for PML/RARA to show distribution of wild type or mutant PML/RARA in nucleus, and with DAPI to visualize nuclei (top). Mouse PML was not recognized by the rabbit anti-PML. HPCs were harvested from the first round colonies. Representative of three independent experiments. Scale bar, 10 µm. Western blotting analysis of fusion proteins with anti-RARA in transduced NIH-3T3 cells (C) or mouse HPCs (D).