CXCL12 induces connective tissue growth factor (CTGF) expression in WI-38 cells.

<p>A, Cells were treated with various concentration of CXCL12 for 2 h. Whole-cell lysates were prepared and immunodetected with specific antibodies for the CTGF or α-tubulin. Data are presented as the mean ± S.E. of three experiments. *<i>p</i><0.05, compared to the control group without CXCL12 treatment. B, Cells were treated with CXCL12 (10 ng/ml) for 0∼24 h, and then immunodetected with CTGF- or α-tubulin-specific antibodies. Data are presented as the mean ± S.E. of three experiments. *<i>p</i><0.05, compared to the group without CXCL12 treatment. C, Cells were transfected with 0.5 µg of CTGF-Luc and 0.1 µg of pBK-CMV-Lac Z for 24 h and then stimulated with 3∼30 ng/ml of CXCL12 for another 16 h. Cells were harvested for a luciferase activity assay. Data are presented as the mean ± S.E. of four experiments. *<i>p</i><0.05, compared to the group without CXCL12 treatment. D, Cells were pretreated with 3 µM actinomycin D (Act.D) and 3 µM cycloheximide (CHX) for 30 min and then stimulated with CXCL12 (10 ng/ml) for another 2 h. Whole-cell lysates were prepared and immunodetected with specific antibodies for the CTGF or α-tubulin. Data are presented as the mean ± S.E. of three experiments. *<i>p</i><0.05, compared to CXCL12 treatment.