Involvement of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinse (JNK) in CXCL12-induced c-Jun phosphorylation or connective tissue growth factor (CTGF) expression in WI-38 cells.

<p>A, Cells were treated with CXCL12 (10 ng/ml) for 0∼60 min. Whole-cell lysates were prepared and immunodetected with specific antibodies for phospho-c-Jun Ser 63 or c-Jun. Data are presented as the mean ± S.E. of three experiments. *<i>p</i><0.05, compared to the control without CXCL12 stimulation. Cells were pretreated with 10 and 30 µM PD98059 (B) or 10 and 30 µM SP600125 (C) for 30 min and then stimulated with CXCL12 (10 ng/ml) for another 20 min. Whole-cell lysates were prepared and immunodetected with specific antibodies for phospho-c-Jun Ser 63 or c-Jun. Data are presented as the mean ± S.E. of three experiments. *<i>p</i><0.05, compared to CXCL12 treatment. D, Cells were pretreated with SP600125 (3∼30 µM) for 30 min and then stimulated with CXCL12 (10 ng/ml) for another 2 h. Whole-cell lysates were prepared and immunodetected with specific antibodies for the CTGF or α-tubulin. Data are presented as the mean ± S.E. of three experiments. *<i>p</i><0.05, compared to CXCL12 treatment.