VP19C binds non-specifically to dsDNA probes.

<p>Three different nucleic acids were used in the EMSA; HSV-1 DNA (BamH1 K, 5.9 kb; lanes 1–6), plasmid pUC19 (2.7 kb; lanes 11–14) that was linearized by EcoR1 and an oligonucleotide (28mer; lanes 7–10) to test the specificity of the binding. All DNA molecules were 5′-end-labeled with [γ-³²P] ATP. In lanes 1, 7 and 11, dsDNA or oligonucleotide probe alone was added to the assay (1.5 ng). In lanes 2-4 the DNA probe was incubated with increasing concentrations of VP19C (1.6 µg, 3.2 µg and 6.4 µg respectively) in NP-complex forming buffer. Similarly in lanes 8 and 12, 1.6 µg of VP19C was used and in lanes 9 and 13, 6.4 µg of VP19C was used. Lane 6 conditions were the same as lane 4 but the NP-complex forming buffer was free of ZnCl₂. In lanes 5, 10 and 14 the DNA-probe was incubated with 6.4 µg of purified GST.