Systemic memory Th-cytokine profiles.

<p>(A–C) Splenocytes of naive and infected mice were collected 66 days p.i. and in vitro restimulated with Prn, FHA or Ptx for 8 days. The percentages of IFNγ-, IL-17A-, and IL-5-producing CD3⁺CD4⁺CD44⁺ T-cells were determined using ICS. (D–F) Cytokine levels in supernatant after 7 days of stimulation were determined by using a MIA. Results (mean of n = 6) are corrected for the background level in the presence of medium as control. Statistical differences between the groups were detected for the ICS with a non-parametric Mann-Whitney test and for the MIA with a Student t-test on the log-transformed data. ** = <i>p</i><0.01, *** = <i>p</i><0.001, **** = <i>p</i><0.0001.