(A and G–I) FISH and (B–F) CARD-FISH images of microbial cells cultivated in the DHS bioreactor.

<p>Photomicrographs of DAPI-stained cells (left) and epifluorescence (right) showing identical fields. (A) Chain-forming ANME-1-350-stained cells in the 2,013-day sample (Alexa Fluor 488, green). (B) An ANME-2a-647-stained coccoid-shaped cell in the 2,013-day sample (Fluorescein, green). (C and D) Color overlay of ANME-2c-760- (Fluorescein, green) and EUB338-stained cells (Alexa Fluor 594, red) in the 903-day sample. (E) A MBGB-380-stained coccoid-shaped cell in the 2,013-day sample (Fluorescein, green). (F) A MBGD-318-stained rod-shaped cell in the 2,013-day sample (Fluorescein, green). (G) A MCOCID442-stained coccoid-shaped cell in the 2,013-day sample. (H) Mγ669-stained coccoid-shaped cells in the 2,013-day sample (Alexa Fluor 488, green). (I) UncGAM731-stained irregular rod-shaped cells in the 2,013-day sample (Alexa Fluor 488, green). Bars represent 10 µm.