FDA/DAPI double staining of cryogel sections after 12 days in culture.

<p>Fig. 7A shows the bright field image of AVP cryogel with FDA/DAPI double stained NIH3T3 fibroblasts at day 12. Fig. 7B shows FDA stained viable cells (greenish fluorescence) and Fig. 7C shows DAPI stained viable and non-viable cells (bluish fluorescence) in AVP cryogel. Fig. 7D is a merged RGB image of Figs. 7A-C, respectively where cyan color indicates cell viability while non-viable cells show blue fluorescence alone. Figs. 7E, F are merged RGB images of double stained AADP and NVP cryogels, respectively (day 12). The merged images (Figs. 7D-F) of all the three cryogels are rich in cyan colored portions indicating their ability to support cell adhesion and viability.