Loss of HEN2 results in accumulation of polyadenylated antisense transcripts.

<p>3′ RACE-PCR. A diagram of the At5g44306 locus is shown at the top. A blue arrow represents the At5g44306 mRNA. Red bars above the diagram represent probes detected in the tiling analysis. The location of the primer used for 3′ RACE PCR is indicated by a green arrow above the diagram. Each of the orange horizontal bars below the diagram represents a polyadenylated clone obtained from the indicated sample. 28 of 32 clones obtained from <i>hen2</i> samples and 3 of 32 clones obtained from <i>mtr4</i> samples corresponded to antisense transcripts of 67 to 208 nt lenght. 0 of 32 clones obtained from WT samples corresponded to the target sequence.