ISH and ICC of <i>A. californica</i> CNS.

<p>Representative photomicrographs of ISH (purple; B, D, F, J) or ICC (brown; A, C, E, G, H, I, K, L, M) staining in the CNS of <i>A. californica</i>, including abdominal (A, B, L, M), buccal (K), cerebral (C, D, I, J), pleural (E, F), and pedal (H) ganglia, and bag cell neurons (G). Preadsorption with ap-AKH (M) completely abolished ap-AKH-ir signal compared to an adjacent section (L). Green = methyl green nuclear counterstain. Solid arrows denote ap-AKH-ir or ap-AKH transcript-positive neuronal cell bodies; arrow pairs of the same shape in panels A/B, C/D, E/F, and L/M point to identical neurons in adjacent sections. Dashed arrows in panels A, C, E, G, H, I, and K point to ap-AKH-ir fibers in the neuropil regions of ganglia. Dashed arrow in panel J points to <i>ap-AKH</i> transcript-positive fibers. Dashed outline in panel L surrounds ap-AKH-ir fibers in the neuropil region of the abdominal ganglia, and in M surrounds the same region of neuropil devoid of signals. Scale bars = 50 µm.