Immunostaining of apUII-positive neurons and fibers in the Aplysia CNS.

<p>(<b>A</b>) The distribution of immunostained neurons in the buccal ganglion is similar to the <i>in-situ</i> hybridization (ISH) staining distribution shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048764#pone-0048764-g002" target="_blank">Figure 2B</a>. Abbreviations, rBG: rostral surface of the buccal ganglion; cBG: caudal surface of the buccal ganglion. Arrowheads point to individual stained neurons. <b>(B</b>) The distribution of immunostained neurons in the cerebral ganglion is similar to the ISH staining distribution shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048764#pone-0048764-g002" target="_blank">Figure 2B</a>. Abbreviations, dCG: dorsal surface of the cerebral ganglion; vCG: ventral surface of the cerebral ganglion; CPC cerebral-pedal connective; A, C, F, neuronal clusters. (<b>C</b>) The distribution of immunostained neurons in the pedal, pleural and abdominal ganglia. Abbreviations, rdPPG: right dorsal pleural-pedal ganglia; ldPPG: left dorsal pleural-pedal ganglia (for these two images, the pleural ganglia are on the left); rvPPG: right ventral pleural-pedal ganglia; lvPPG: left ventral pleural-pedal ganglia (for these two images, the pleural ganglia are on the right); dAG: dorsal abdominal ganglion; vAG: ventral abdominal ganglion. Arrowheads point to stained neurons. Calibration bar for all panels : 200 µm.