The native PAGE of the R-PC and AP sample performed in the HEPES−Imidazole buffer.

<p>The PAGE had a resolving gel of 7% in pH 6.5 HEPES−Imidazole buffer and a stacking gel of 4% in pH 5.4 Bis-tris−Acetic acid buffer; the Tricine−Bis-tris electrode buffers in pH 6.3 were employed as anode and cathode solutions. (a) the bands in natural colors, (b) the bands of native state in grey scale without red filter, (c) the bands of native state in grey scale with red filter and (d) the bands in fluorescence under UV-light at 365 nm. The R-PC and AP sample used in the PAGE was the R-PC fraction from the ion exchange chromatography.