DENV infection induces changes in the distribution of secreted α-enolase isoforms.

<p>HepG2 cells were either mock-infected or infected with DENV (MOI = 4) for 24 h. Conditioned medium was collected and concentrated and the proteins were analyzed by two-dimensional gel electrophoresis (2-DE). (A) Gels stained with colloidal Coomassie blue. The circles indicate the region for α-enolase separation, which was selected accordingly to the theoretical molecular mass and isoeletric point of the protein. (B) Immunodetection of α-enolase isoforms on the 2-DE Western blot. α-enolase spots were detected in the region correspondent to the circles in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088314#pone-0088314-g003" target="_blank">Figure 3A</a>. The figure shows a representative blot of 4 independent experiments (C) Densitometric analysis of α-enolase spots. Graph shows mean ± SEM from 4 independent experiments. Differences between spots from mock and DENV-infected cells were assessed using repeated measures two-way ANOVA followed by Bonferroni <i>post hoc</i> test. Statistical significance compared with the control is indicated by asterisks (***<i>p</i><0.001,*<i>p</i><0.001).