Influence of anti-prion compounds on the amount of PrP-res.

<p>(A) ScN2a-3-22L cells grown on 12-well plates were cultured in the presence or absence of mAb 44B1, PPS, CPZ, or U18666A at the indicated concentration for 72 h. The samples were subjected to immunoblotting and dot-blotting for PrP-res detection or β-actin detection for endogenous control. Representative blots for each compound are shown on the left. The graph on the right shows PrP-res levels relative to the control samples. The means and standard deviations (SDs) of four independent experiments (PrP-res was detected by dot-blotting) are indicated. Graphs on the upper right show the logistic curve fitted to the data of PrP-res levels by dot-blotting (B) ScN2a-3-22L cells were cultured with anti-prion compounds at the EC₆₅ (mAb 44B1, 0.4 µg/ml; PPS, 0.1 µg/ml; CPZ, 10 µM; U18666A, 5 µM) for the indicated time and subjected to dot-blotting for PrP-res. Representative dot-blotting is shown on the left, and the graph on the right shows the levels of PrP-res relative to the samples from ScN2a-3-22L cells cultured without anti-prion compounds for 72 h. The means and SDs of four independent experiments are depicted. Asterisks indicate a significant difference compared with the control at the same time point (Student’s <i>t-</i>test, <i>p</i><0.05).