Equilibrium dissociation curves for select aptamers.

<p>GII.2. SMV VLPs (3 µg/ml) were screened with different concentrations (10 nM, 100 nM, 500 nM, 1 µM, 2 µM) of each aptamer using ELASA. To estimate K_d, plots of the T/N ratios (absorbance at 450 nm) as a function of the aptamer concentration were fitted to a non-interacting binding sites model with the equation <i>Y</i> = Bmax <i>X</i>/Kd+<i>X</i>. The negative controls consisted of PBS. Results correspond to aptamers SMV 19 (A), SMV 21 (B), SMV 25 (C) and SMV 26 (D).