<p>A constant number of Protein A/Strep-MB in each assay and a concentration series of the fluorescein-labeled aptamer were used. The dissociation constant (<i>K</i><sub>D</sub>) of 1.06 ±0.2 μM was calculated by nonlinear regression analysis.</p
Registration of fluorescence anisotropy (FA) allows for characterizing the interactions of ligands w...
<p>Biacore X100 / sensor chip CAP / ligand: 3'-biotinylated truncated aptamer PA#2/8[S1-58] with imm...
The thrombin-binding 15mer and 29mer ssDNA aptamers are a widely used model system. Despite their ub...
<p>Binding curves for the interactions of fluorescently labeled aptamer PA#2/8 (A, D) or its truncat...
<p>GII.2. SMV VLPs (3 µg/ml) were screened with different concentrations (10 nM, 100 nM, 500 nM, 1 µ...
<p>The Binding affinity of AraHH001 was determined by a flow cytometry using FITC-AraHH001 to mTECs ...
Aptamers that bind small molecules can serve as basic biosensing platforms. Evaluation of the bindin...
The dissociation constant of aptamer binding to C4-HSL by saturation binding model.</p
<p>Biacore X100 / sensor chip CAP / ligand: biotinylated native Protein A with immobilization level ...
<p>(A) Biotinylated aptamers immobilized on an NLC sensor chip and interacting with various amounts ...
<p>Biacore X100 / sensor chip CAP / ligand: 3'-biotinylated aptamer PA#2/8 with immobilization level...
<p>Bead-based binding assays were performed using Protein A/Strep-MB and fluorescein-labeled ssDNA. ...
<p>Binding between the Orai1 peptide and the biotin-labelled Aptamer Y1 was evaluated by measuring t...
<p>A) Ten nM of fluorescein labeled βB-1 was mixed with 100 nM of β-BuTx, BSA, α-BuTx and 2.1 µg/mL ...
<p>Cells were incubated with different SL<sub>2</sub>-B aptamer concentrations ranging from 0 to 100...
Registration of fluorescence anisotropy (FA) allows for characterizing the interactions of ligands w...
<p>Biacore X100 / sensor chip CAP / ligand: 3'-biotinylated truncated aptamer PA#2/8[S1-58] with imm...
The thrombin-binding 15mer and 29mer ssDNA aptamers are a widely used model system. Despite their ub...
<p>Binding curves for the interactions of fluorescently labeled aptamer PA#2/8 (A, D) or its truncat...
<p>GII.2. SMV VLPs (3 µg/ml) were screened with different concentrations (10 nM, 100 nM, 500 nM, 1 µ...
<p>The Binding affinity of AraHH001 was determined by a flow cytometry using FITC-AraHH001 to mTECs ...
Aptamers that bind small molecules can serve as basic biosensing platforms. Evaluation of the bindin...
The dissociation constant of aptamer binding to C4-HSL by saturation binding model.</p
<p>Biacore X100 / sensor chip CAP / ligand: biotinylated native Protein A with immobilization level ...
<p>(A) Biotinylated aptamers immobilized on an NLC sensor chip and interacting with various amounts ...
<p>Biacore X100 / sensor chip CAP / ligand: 3'-biotinylated aptamer PA#2/8 with immobilization level...
<p>Bead-based binding assays were performed using Protein A/Strep-MB and fluorescein-labeled ssDNA. ...
<p>Binding between the Orai1 peptide and the biotin-labelled Aptamer Y1 was evaluated by measuring t...
<p>A) Ten nM of fluorescein labeled βB-1 was mixed with 100 nM of β-BuTx, BSA, α-BuTx and 2.1 µg/mL ...
<p>Cells were incubated with different SL<sub>2</sub>-B aptamer concentrations ranging from 0 to 100...
Registration of fluorescence anisotropy (FA) allows for characterizing the interactions of ligands w...
<p>Biacore X100 / sensor chip CAP / ligand: 3'-biotinylated truncated aptamer PA#2/8[S1-58] with imm...
The thrombin-binding 15mer and 29mer ssDNA aptamers are a widely used model system. Despite their ub...