IL-4 upregulates Eomes in memory CD8⁺ T cells in an Akt-dependent manner.

<p>A) <i>Right</i>, Flow cytometric analysis of Eomes expression in WT peripheral CD8⁺ T cells. <i>Left</i>, Percentage of Eomes⁺ cells among total live CD8⁺ T cells after culture for 20 h in the indicated conditions (n = 6, 3 independent experiments). B) <i>Top</i>, Flow cytometric analysis of Eomes expression in live TCRβ⁺ CD8⁺ T cells from WT naïve (CD44⁻CD62L⁺) and memory (CD44⁺CD62L⁺) CD8⁺ splenocytes cultured in indicated conditions for 20 h. <i>Bottom</i>, Percentage of Eomes⁺ cells among total live CD8⁺ T cells (n = 8 for naïve CD8⁺ cells, 3 independent experiments; n = 4 for memory CD8⁺ T cells, 2 independent experiments). C) <i>Top</i>, Flow cytometric analysis of intracellular Eomes expression in WT memory CD8⁺ T cells treated for 20 h in indicated conditions. <i>Bottom</i>, Percentage of Eomes⁺ cells among live memory CD8⁺ T cells (n = 4, 2 independent experiments). Live lymphocyte gate was determined by forward and side scatter gating. Numbers in flow plots represent the percent of the gated population. Graphs show the average percentage of the indicated population and standard error of mean. Statistical significance calculated using Student’s t-test (A, B) or one-way ANOVA with Tukey’s multiple comparison post-test (C).