mTORC1 is partially required for IL-4 regulation of Eomes in CD8SP thymocytes.

<p><i>Top</i>, Flow cytometric analysis of intracellular Eomes expression in WT TCRβ⁺ CD8P thymocytes after culture in the indicated conditions for 20 h. <i>Bottom left</i>, Percentage of Eomes⁺ cells among total CD8SP thymocytes (n = 8, 3 independent experiments). <i>Bottom right</i>, Flow cytometric analysis of intracellular phopho-S6 expression in WT CD8SP thymocytes after culture in indicated conditions for 20 h, representative of n = 5 per group, 2 independent experiments. Numbers in flow plots represent the percent of the gated population. Graphs show the average percentage of the indicated population and standard error of mean. Statistical significance calculated one-way ANOVA with Tukey’s multiple comparison post-test.