1D ¹H-NMR spectra of cyt <i>c</i>.

<p>(A) The high-field shifted region of the native cyt c (down) and its Y67H variant (upper) spectra. (B) The down-field shifted region of the native cyt <i>c</i> (down) and its Y67H variant (upper) spectra. The peaks in 1D ¹H-NMR spectrum were assigned as (1a) His18 Hε1, (2a) His71 Hε1, (3a) His71 Hδ2, (4a) heme 8-CH₃, (5a) His18 Hδ2, (6a) heme 3-CH₃, (7a) heme 5-CH₃, (8a) His18 Hβ2, (9a) heme 1-CH₃ and (10a) heme 7-Hα2, respectively. The peaks in 1D ¹H-NMR spectrum of native cyt <i>c</i> were assigned as (1b) His18 Hε1, (2b) Met80 ε-CH₃, (3b) Met80 Hγ, (4b) heme 8-CH₃, (5b) heme 3-CH₃, (6b) His18 Hδ2, (7b) heme 7-Hα2, (8b) His18 Hβ2, (9b) heme 7-Hα1 and (10b) His18 Hδ1, respectively.