Unk expression is regulated by <i>Tsc1</i> in differentiating photoreceptors.

<p>(A) A confocal projection of a wild-type eye disc stained for Unk protein expression. Unk is expressed throughout the disc, but its expression is stronger posterior to the morphogenetic furrow (MF, arrow). Scale bar: 50 µm. (B) High magnification single confocal section of Unk expression (red) in differentiating photoreceptors. Unk has a cytoplasmic, partially punctate distribution. Prospero expression marking R7 and cone cells is shown in green. Arrowheads mark examples of puncta. N: nucleus. Scale bar: 10 µm. (C–D) Unk expression (white in (C′),(D′) and red in (D)) is decreased in <i>Tsc1^Q600X</i> mutant clones posterior to the MF (arrows) both in photoreceptors (C, showing apical level) and photoreceptor precursor cells (D, showing basal level), but not in clones anterior to the MF (arrowheads in (D)). Prospero expression (red in (C)) marks R7/cone cells. Mutant clones are marked by loss of GFP expression (green) in (C) and (D). Anterior is to the left.