Protein thiols, GSH and GSSG levles in bone marrow cells.

<p>(<i>A, B)</i> Intracellular reduced protein thiols (A), and GSH/GSSG levels (B) in crude BMCs, Lin(−) cells and BMDDCs. Intracellular reduced thiol and GSH levels were measured by means of a sulfhydryl-specific fluorescent probe; intracellular GSSG levels were determined based on the reduction of GSSG in the presence of glutathione reductase and NADPH and on measurement of NADPH fluorescence decrease. Values are means (±<i>SD</i>) from at least three independent experiments, with asterisks (*) indicating statistical significant differences between (<i>p</i><0.05). (<i>C)</i> Representative MALDI-MS images of GSH and GSSG in sectioned femur showing bone marrow distribution in WT and <i>Gstp1/p2</i>^−/− mice. From left to right: scanned image of matrix sprayed MALDI slide of mouse femur with bone marrow; corresponding images of: GSH ions at m/z = 306.08 and GSSG ions at m/z = 611.14. Color heat map of the data points in the GSH and GSSG images represent averaged individual ion signal intensities of the spots.