Inhibition of IFN-γ secretion by MHC-I in NKL and human activated NK cells.

<p>Exponentially growing NKL cells (<b>A and B</b>) were co-cultured with P815 cells at 1∶1 E/T ratio as described in <i>Materials and Methods</i>. (<b>A</b>) IFN-γ secretion is efficiently inhibited by anti-CD94 mAb in all cases. Anti-MHC-I mAb partially inhibits the secretion of IFN-γ induced by CD16, NKp46 and 2B4. Figure shows (<b>A</b>) one representative assay and (<b>B</b>) percentage of inhibition (mean ±SD) from the four experiments performed. (<b>C and D</b>) IFN-γ secretion by purified quiescent human activated primary NK cells is inhibited by anti-MHC-I mAb. Panel <b>C</b> shows one representative assay out of five (three different donors), and <b>D</b> the percentages of inhibition (mean ±SD) of anti-MHC-I and anti-NKG2A mAb. ND: not determined.