Activation of IFN-γ secretion by NK cells co-cultured with various combinations of poly I:C–stimulated BMDCs and supernatants from these BMDCs

BMDCs from various genotypes (indicated along the x axis of graph) were activated with poly I:C and then supplemented with supernatants exchanged from similarly activated BMDCs. Genotypes of DCs from which supernatants were derived are indicated by individual columns (gray, WT; white, 15KO; black, RαKO; checkered, mixture of 15KO and RαKO). ELISA quantitation of NK cell IFN-γ secretion is indicated on the y axis. Note that WT DCs activate NK cells regardless of the type of supernatant added. Note also that WT DC–derived supernatants containing IL-15–sIL-15Rα complexes fail to support or augment NK cell activation.<p><b>Copyright information:</b></p><p>Taken from "IL-15Rα chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation"</p><p></p><p>The Journal of Experimental Medicine 2008;205(5):1213-1225.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2373851.</p><p>