Representive Western blot (A, D) and densitometric quantification of eNOS uncoupling (B, E) and phospho-p66^Shc protein (C, F) expression in HAEC treated with oxLDL within sixty minutes (A, B, C) or for twenty four hours (D, E, F).

<p>eNOS uncoupling was presented as ratio of dimer/monomer form of eNOS. The phosphorylation of p66^Shc was normalized to total p66^Shc protein and total p66^Shc was normalized to α-tubulin. Results are presented as means±SEM; n = 6. * p<0.05 vs. cells at 0 minutes or cells under control condition.