Effect of stretch-induced eNOS activation and NO production on WPB exocytosis and leukocyte adhesion.

<p>(A) Quantitative analysis of stretch-induced vWF release from HUVECs pretreated with SNAP (50 µM), L-NAME (1 mM) or vehicle (DMSO). (B) <i>Left</i>: quantitative analysis of stretch-induced vWF release from HUVECs expressing scrambled (Scr) or eNOS-targeting (eNOS KD) shRNAs. <i>Right</i>: Western blots of the knockdown efficiency of shRNAs targeting eNOS in HUVECs. (C) <i>Left</i>: HL-60 cell adhesion to HUVEC monolayers after stretch, pretreated with SNAP (50 µM), L-NANE (1 mM) or vehicle (DMSO). <i>Right</i>: quantitative analysis of HL-60 adhesion. (D) <i>Left</i>: stretch-induced HL-60 cell adhesion to HUVECs expressing scrambled (Scr) or eNOS-targeting (eNOS KD) shRNAs. <i>Right</i>: quantitative analysis of HL-60 adhesion. Results are representative of 3 individual experiments and expressed as mean ± S.D. (n = 4). **P<0.01.