Restoration of physiologically relevant amounts of CXCL1 during infection restores neutrophil levels and survival of HIF^C mice.

<p>(A) BMDNs migration from littermate (WT) and HIF^C mice in the presence of 12 hr BALF from HIF, HIF+ 600 pg rCXCL1, and WT mice inoculated with 7×10⁷ conidia was measured using a 3 µm transwell chamber. Immune competent WT and HIF^C mice received 7×10⁷ conidia (Af) i.t. and were treated with 50 ng rCXCL1 4 hr post conidial challenge and monitored for (B) survival (p<0.002, 2 experiments: 12 mice total), (C) fungal burden at 36 hrs (4 biological, 3 technical reps), and (D) FACs analysis of BALF cellularity including: CD11b+Ly6G+ neutrophils, CD11b+Ly6G- monocyte-like, and CD11c+ macrophages, (N = 4 per group, representative of two experiments). Representative histology of H&E and GMS stained sections of the HIF^C+CXCL1 mice (E) at 12 hr and 24 hr and H&E sections of (F) 12 and 24 hr vasculature (20×). (G) WT and HIF^C BMDNs were stimulated with PBS or 60 pg rCXCL1 for 22 hrs and AnnexinV/PI staining was conducted using FACs (combined two biological experiments). Av+PI+ (necrotic), Av+PI− (apoptotic), Av−PI− (normal).